Spatiotemporal distribution of microplastics in true frogs (Ranidae:Pelophylax) populations from Türkiye.

Microplastics (MPs), which are of increasing concern in almost all ecosystems, continue to be an environmental threat. In the present study, the presence of MPs is investigated by using the gastrointestinal tracts (GITs) obtained from the true frog individuals sampled in the past years. The sample consists of a total of 146 individuals obtained from various regions of Türkiye. The results show that MPs were found in 87 adult frog individuals. The most predominant shape of MPs found was fiber, with a predominance of transparent color, and ethylene-vinyl acetate (EVA) was also the most abundant microplastic material. The highest amount of MPs among adult frog populations were found in the Denizli province. The size of MPs ranged between a minimum of 66 and a maximum of 3770 µm. In addition, no relationship was found between the body length and weight of the frogs and the sizes of MPs. Historically, most MPs were found in samples from 1990 and the highest content in terms of both color and material was detected in 1990. MPs were detected in almost all the years studied, but microplastic abundance varied from year to year. These results can be explained by the number of samples, habitat, and feeding behavior of adult frogs.

Climatic envelopes of the genus Lacerta Linnaeus, 1758 in Türkiye: an application of ecological niche modeling.

Six species belonging to the genus Lacerta live in Türkiye. In this study, both present and future potential distribution maps were created based on occurrence data and climatic variables for these six species. Two scenarios for future projections (shared socioeconomic pathways, SSPs,: 245 and 585) and two timeframes (2041-2060 and 2081-2100) were used. The present and future potential distributions of these species were compared. As a result, it was predicted that the distribution ranges in the six species will expand in the future, and this expansion has revealed new environments.

Easternmost distribution of Bufo bufo (Linnaeus, 1758) in Türkiye: implications for the putative contact zone between B. bufo and B. verrucosissimus.

The geographic range of a species is crucial for obtaining information on the exact distribution of the species. The geographic data are important for delimiting distinct species or exploring the degree of differentiation among different populations of a species. The local details of species boundaries facilitate the study of the importance of phylogeographic background, secondary contacts, and hybrid zones, along with the relations between the species and its extrinsic environmental factors. In the present study, the range boundaries of Bufo bufo and Bufo verrucosissimus in the north-eastern region of Türkiye were delineated using an integrative taxonomic approach that utilized a combination of molecular and morphological data. According to the mtDNA results of the present study, B. bufo inhabits a single distribution from Iyidere town to Çayeli town in Rize, while B. verrucosissimus is distributed from Savsat town of Artvin to Ardesen town in Rize. In addition, the two species coexist in Pazar, Hemsin, and Çamlihemsin towns in Rize. The demographic analyses indicated a distinct population expansion for the B. verrucosissimus species after the Last Glacial Maximum, while the same did not occur for B. bufo. The univariate and multivariate statistical analyses conducted for the morphological data of the two species corroborated the presence of a putative contact zone between B. bufo and B. verrucosissimus. In summary, the present study resolved the non-distinct geographic boundaries between B. bufo and B. verrucosissimus species and also revealed the easternmost distribution of B. bufo in Türkiye. In addition, important evidence on the putative contact zone between the two species was indicated using an integrative taxonomic approach.

Revising the taxonomy of Darevskia valentini (Boettger, 1892) and Darevskia rudis (Bedriaga, 1886) (Squamata, Lacertidae): a Morpho-Phylogenetic integrated study in a complex Anatolian scenario.

Revealing biodiversity allows the accurate determination of the underlying causes of many biological processes such as speciation and hybridization. These processes contain many complex patterns, especially in areas with high species diversity. As two of the prominent zoogeographic areas, Anatolia and Caucasus are also home to the genus Darevskia, which has a complex morphological structure and parthenogenetic speciation. Darevskia valentini and D. rudis are two largely distributed taxa of this genus, both of which have a controversial taxonomic delimitation. Here we performed both a highly detailed morphological comparison and a molecular evaluation for the populations in both species groups. The most comprehensive taxonomic revision of this complex was carried out to determine the cases where the data obtained were compatible or not with each approach. As a result of the obtained outputs, it seems that D. spitzenbergerae stat. nov., D. mirabilis stat. nov. and D. obscura stat. nov. should be accepted as the species level, this later with subspecies D. o. bischoffi comb. nov. and D. o. macromaculata comb. nov.. Also, we propose two new taxa: D. josefschmidtleri sp. nov. and D. spitzenbergerae wernermayeri ssp. nov.. It has also been shown that "lantzicyreni" subspecies belong to D. rudis instead of D. valentini. The extensive revision has contributed to subsequent studies to more accurately understand the past histories of species in the genus Darevskia.

Potential habitats of an alien species (Asterias rubens Linnaeus, 1758) in the Black Sea: its current and future distribution patterns.

The Atlantic common starfish, Asterias rubens, has arrived firstly at the Marmara Sea in 1996 and to the Black Sea in 2007. In this study, we have exhibited the possible potential distribution of Asterias rubens throughout the Black Sea. For this, we predicted and determined the present and future distributions, and habitat preferences of this starfish in the Black Sea using environmental variables. The ecological niche modeling was used to detect the suitable habitat of A. rubens. In the current model, shallow areas seem to be the suitable habitat for A. rubens. However, this trend may change in the future distribution pattern. For the future projection, two representative concentration pathways (RCPs) that are a greenhouse gas concentration was used: RCP2.6 that is likely to keep global temperature rise below 2 °C by 2100 and RCP8.5 that will happen approximately 5 °C in range of global mean temperature increase in 2100 from pre-industrial baseline. According to RCP2.6 scenarios as well as the RCP8.5 scenario in 2040-2050, the suitable habitats in the Black Sea will probably decrease due to climate change. The most suitable habitats in these scenarios will remain the western and southern coasts of the Black Sea because these areas will be less affected by the change in the climate. In contrast, for the 2090-2100 periods of the RCP8.5, there will likely be a significant unsuitable habitat throughout the Black Sea. Therefore, the suitable habitat for A. rubens will be restricted to the western and southern coasts of the Black Sea.

Sexual size and shape dimorphism in Turkish common toads (Bufo bufo Linnaeus 1758).

Differences in male and female traits (sexual dimorphism [SD]) are widespread in animals. Dimorphism in morphological characters evolves under the effect of environmental and genetic factors and is shaped by natural and sexual selection. In this study, intersexual differences in size and shape in common toad, Bufo bufo, populations in Turkey were investigated. For this, linear measurements of 27 body-related morphometric characters in a sample of 140 individuals (70 males and 70 females) were compiled. The data were analyzed using univariate and multivariate statistical methods. The results show SD in body size with females having larger body size, a trait related with fecundity and thus probably under sexual selection. Body shape differences, which are associated with head width and dorsal head, are likely to prey size. Male-biased differences observed in nasal characters and tympanum may also be associated with sexual selection (male-male competition). Our findings are consistent with previous studies of major of Anura and support a role for selection pressures acting differentially upon individuals from both sexes, resulting in the evolution of sexually dimorphic traits.

Impact of various ecological parameters on the life-history characteristics of Bufotes viridis sitibundus from Turkey.

In this study, we used the skeletochronology method to estimate various growth parameters, such as age structure, minimum and maximum life span, age of sexual maturity, and the relationship between body size and weight of eight different populations of the variable toad, Bufotes viridis sitibundus, in Turkey. Further, we determined the relationship between these parameters and ecologic factors using the partial Mantel test. A significant difference was found among the populations with respect to age, body size, and body weight in both males and females. On average, the maximum life span was recorded as 10 years for males and 11 years for females. In the studied populations, the average age of sexual maturity ranged between 2 and 4 years for both sexes. Sexual dimorphism in terms of snout-vent length (SVL) was not observed between males and females in all the populations. Toads from the higher altitudes tended to be significantly larger, older, and heavier than those from lower altitudes. We concluded that altitude and temperature have an impact on the growth rate, body size, and body mass.

Characterization of microplastic pollution in tadpoles living in small water-bodies from Rize, the northeast of Turkey.

Microplastic pollution is a growing problem for Turkey and other countries, but most studies focus on the pollution in oceans and seas. To understand the relationship between microplastics, fresh water, and terrestrial environment, we examined Pelophylax ridibundus and Rana macrocnemis tadpoles that can inhabit a wide range of both terrestrial and aquatic habitats, ecoregions and elevations, and that are members of Ranidae family. We characterized microplastics (MPs) in sediments, surface water, and tadpoles from the Rize province in northeastern of Turkey. The content of MPs in sediments, surface water, and tadpoles, ranged 64.17-472.1 items/kg, 1-13 items/L and 302.62-306.69 items/g, respectively. In sediment samples, polypropylene (PP) and polyethylene (PE) were the dominant pollutants; whereas, nylon and polyethylene terephthalate (PET) were found in surface water. In tadpoles, PET, nylon, and polyacrylic were the dominant MPs.